Background: Recombinant proteins are usually required in laboratories interested in the protein but not in the\nproduction process itself. Thus, technical equipment which is easy to handle and straight forward protein\nproduction procedures are of great benefit to those laboratories. Companies selling single use cultivation bags and\nbioreactors are trying to satisfy at least part of these needs. However, single-use systems can contribute to major\ncosts which might be acceptable when ââ?¬Å?good manufacturing practicesââ?¬Â are required but not acceptable for most\nlaboratories facing tight funding.\nResults: The assembly and application of a simple self-made ââ?¬Å?smart sustainable bottleââ?¬Â (SSB) system for E. coli based\nprotein production is presented. The core of the SSB system is a 2-L glass bottle which is operated at constant\ntemperature, air flow, and stirrer speed without measurement and control of pH and dissolved oxygen. Oxygen\ntransfer capacities are in the range as in conventional bioreactors operated at intermediate aeration rates and by far\nexceed those found in conventional shaking flasks and disposable bioreactors. The SSB system was applied for the\nproduction of various recombinant proteins using T7-based expression systems and a defined autoinduction\nmedium. The production performance regarding amount and solubility of proteins with robust and delicate properties\nwas as good as in state-of-the-art stirred tank commercial bioreactors.\nConclusions: The SSB system represents a low cost protein production device applicable for easy, effective, and\nreproducible recombinant protein production.
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